Preserving vegetation is crucial to develop a vineyard worthy of the name and produce wines of excellence.  As an HVE 3-certified estate since the 2020 vintage, Domaine Maire & Fils implement an annual programme designed to promote the biodiversity of its winegrowing heritage.  The bare soil is sown with nectar source plants to prepare the soil for 3 to 4 years before new vines are planted. These flower meadows also nourish the six beehives installed on the Sorbief plot, which produced their first honey in 2022. The initial hedgerow planting programme launched in 2020 will be extended to a larger scale in 2024,  with an additional 7 hectares will be planted in Sorbief and Barodottes. Over 450 m of hedges consisting of 10 to 80 plants of different species will be represented among which dog roses, buckthorns, viburnums, black elders, hawthorns, blackthorns, dogwoods, European spindles, wild pear and apple trees and pussy willows. The lower part of the plot will be landscaped with a pond to accommodate amphibians and other fauna, once again contributing to the biodiversity of the estate.

The estate is also involved with the Observatoire Agricole de la Biodiversité* (National Agricultural Observatory for Biodiversity) programme, participating in a series of counting initiatives: earthworms, butterflies, wild bees and bats, some of which are indicators of how ecosystems operate, while others are invaluable in protecting vineyards.